The PPA1 Antibody (CAB14050) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By targeting the PPA1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and metabolic research.PPA1, also known as pyrophosphatase 1, plays a crucial role in maintaining cellular energy balance by catalyzing the hydrolysis of inorganic pyrophosphate.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PPA1 Antibody
SKU:
CAB14050
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP, PP1, IOPPP, HEL-S-66p, SID6-8061, PPA1
Positive Sample:
Raji, SKOV3, U-251MG, SW620, 293T, HepG2, Mouse liver, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
37kDa
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Purification Method
Affinity purification
Gene ID
5464
RRID
AB_2760905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPA1 Rabbit pAb (CAB14050) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
