Description
PPARA Polyclonal Antibody (CAB24835)
The PPARA Polyclonal Antibody (CAB24835) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the PPARA protein, this antibody allows for the detection and analysis of PPARA expression in various cell types, making it ideal for research in metabolic disorders, cardiovascular disease, and diabetes.
This antibody is validated for use in WB applications and has demonstrated reactivity against Mouse samples.
| Product Name: | PPARA Polyclonal Antibody |
| SKU: | CAB24835 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||
| Sequence: | ADLK SLAK RIYE AYLK NFNM NKVK ARVI LSGK ASNN PPFV IHDM ETLC MAEK TLVA KLVA NGIQ NKEA EVRI FHCC QCTS VETV TELT EFAK AIPG FANL | ||
| Tested Applications: | WB | ||
| Recommended Dilution: |
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| Synonyms: | PPAR, NR1C1, hPPAR, PPARalpha, PPARA |
| Positive Sample: | Mouse kidney |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 52kDa |
| Observed MW: | 52kDa |
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.
| Purification Method | Affinity purification |
| Gene ID | 5465 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse kidney using PPARA Rabbit pAb (CAB24835) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
