The PPM1G Monoclonal Antibody (CAB20959) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, developed in rabbits, exhibits high specificity and sensitivity in detecting PPM1G in human samples, making it suitable for use in Western blotting and immunohistochemistry studies.PPM1G, also known as protein phosphatase Mg2+/Mn2+ dependent 1G, plays a crucial role in dephosphorylating target proteins and regulating their activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PPM1G Monoclonal Antibody
SKU:
CAB20959
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2947
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP2CG, PPP2CG, PP2CGAMMA, PPM1G
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Lipid-Anchor, Membrane.
Calculated MW:
59kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
Purification Method
Affinity purification
Gene ID
5496
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from various lysates, using PPM1G Rabbit mAb (CAB20959) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
