The PREX1 Polyclonal Antibody (CAB18344) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples and is validated for use in Western blot applications. By specifically binding to the PREX1 protein, this antibody allows for the detection and analysis of PREX1 in a variety of cell types, making it an essential tool for studies in cell biology, cancer research, and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PREX1 Polyclonal Antibody
SKU:
CAB18344
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P-REX1, PREX1
Positive Sample:
T-47D,
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
186kDa
Observed MW:
190kDa
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
Purification Method
Affinity purification
Gene ID
57580
RRID
AB_2862114
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from T-47D cells using PREX1 Rabbit pAb (CAB18344) at 1:3700 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 60 s.
