The PRG4 Antibody (CAB15379) is a high-quality antibody developed for reliable detection and analysis of target proteins. PRG4 (Proteoglycan 4/Lubricin) is a large proteoglycan synthesized by articular chondrocytes and synovial lining cells that functions as a boundary lubricant at cartilage surfaces, contributing to elastic absorption and energy dissipation within synovial joints. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis (CACP) syndrome, characterized by progressive joint destruction.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PRG4 Antibody
SKU:
CAB15379
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MSF, SZP, CACP, HAPO, JCAP, PRG4
Positive Sample:
LO2
Cellular Localization:
Secreted.
Calculated MW:
151kDa
Observed MW:
150kDa
The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10216
RRID
AB_2762284
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from LO2 cells, using PRG4 Rabbit pAb (CAB15379) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
