The PRL Antibody (CAB1618) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. RRID AB_2763630 Gene ID 5617 Swiss Prot Synonym GHA1; PRL
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRL Antibody
SKU:
CAB1618
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GHA1, PRL
Positive Sample:
293F-Prolactin-Avi-His-C
Cellular Localization:
Secreted.
Calculated MW:
26kDa
Observed MW:
24kDa
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. RRID AB_2763630 Gene ID 5617 Swiss Prot Synonym GHA1; PRL
Purification Method:
Affinity purification
Gene ID:
5617
RRID:
AB_2763630
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293F cells and 293F transfected with 293F-Prolactin-Avi-His-C protein, using PRL Rabbit pAb (CAB1618) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human pituitary gland using PRL Rabbit pAb (CAB1618) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
