Coagulation Protein C Monoclonal Antibody (CAB4532)
The Coagulation Protein C Monoclonal Antibody (CAB4532) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbits, is highly specific and reacts strongly with human samples, making it ideal for use in various applications such as Western blotting and immunohistochemistry.Protein C plays a key role in the anticoagulant pathway, serving as a natural anticoagulant by inhibiting blood clot formation. Dysregulation of Protein C has been linked to various thrombotic disorders, making it an important target for research in hemostasis and thrombosis. By using the Protein C Rabbit Monoclonal Antibody, researchers can investigate the expression and function of Protein C in different tissues and cell types, providing valuable insights into its role in maintaining vascular health.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Coagulation Protein C Monoclonal Antibody
SKU:
CAB4532
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1023
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PC, APC, PROC1, THPH3, THPH4, Coagulation Protein C
Positive Sample:
HT-29, Mouse liver
Cellular Localization:
Endoplasmic Reticulum, Golgi Apparatus, Secreted.
Calculated MW:
52kDa
Observed MW:
52kDa
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
Purification Method
Affinity purification
Gene ID
5624
RRID
AB_2863289
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Coagulation Coagulation Protein C Rabbit mAb (CAB4532) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Protein C Rabbit mAb (CAB4532, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
