The PROK1 Monoclonal Antibody (CAB19268) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. RRID Gene ID 84432 Swiss Prot Synonym PK1; PRK1; EGVEGF; PROK1
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PROK1 Monoclonal Antibody
SKU:
CAB19268
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2439
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PK1, PRK1, EGVEGF, PROK1
Positive Sample:
293T, HepG2, Mouse liver, Mouse kidney, Rat ovary, Rat kidney
Cellular Localization:
Extracellular Region.
Calculated MW:
12kDa
Observed MW:
14kDa
The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. RRID Gene ID 84432 Swiss Prot Synonym PK1; PRK1; EGVEGF; PROK1
Purification Method:
Affinity purification
Gene ID:
84432
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Human EG-VEGF/PK1 Rabbit mAb (CAB19268) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded Rat placenta tissue using Human EG-VEGF/PK1 Rabbit mAb (CAB19268) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
