The JARID2 Antibody (CAB9823) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the Jumonji protein, researchers can detect and analyze its expression in various cell types, making it an essential tool for studies in epigenetics, gene regulation, and cancer research.The Jumonji protein, part of the JmjC-domain-containing protein family, functions as a histone demethylase, controlling gene expression by modifying chromatin structure.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
JARID2 Antibody
SKU:
CAB9823
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JMJ, DIDDF, JARID2
Positive Sample:
HL-60, Mouse thymus, Rat heart
Cellular Localization:
Nucleus.
Calculated MW:
139kDa
Observed MW:
160kDa
This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies.
Purification Method
Affinity purification
Gene ID
3720
RRID
AB_2770028
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using JARID2 Rabbit pAb (CAB9823) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
