The PSMB2 Antibody (CAB13630) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically reactive with human samples and has been validated for use in Western blot applications. By binding to the PSMB2 protein, this antibody allows for the detection and analysis of PSMB2 expression in various cell types, making it ideal for studies in molecular biology and cell biology research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PSMB2 Antibody
SKU:
CAB13630
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5690
RRID
AB_2760492
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PSMB2 Rabbit pAb (CAB13630) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Human colon using PSMB2 Rabbit pAb (CAB13630) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg PSMB2 antibody (CAB13630). Western blot was performed from the immunoprecipitate using PSMB2 antibody (CAB13630) at a dilution of 1:1000.
