The PSMB2 Antibody (CAB5483) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly reactive with human samples, making it ideal for Western blot applications. By binding specifically to the PSMB2 protein, this antibody enables accurate detection and analysis in a variety of cell types.The PSMB2 protein is a crucial component of the proteasome complex, responsible for degrading unwanted or damaged proteins in the cell. Research into the function and regulation of PSMB2 is important for understanding cellular processes related to protein degradation, cell cycle control, and immune response.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PSMB2 Antibody
SKU:
CAB5483
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HC7-I, PSMB2
Positive Sample:
HeLa, 293T, SH-SY5Y, Mouse brain, Mouse liver, Rat liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
23kDa
Observed MW:
23kDa
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5690
RRID
AB_2766283
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PSMB2 Rabbit pAb (CAB5483) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using PSMB2 Rabbit pAb (CAB5483) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg PSMB2 antibody (CAB5483). Western blot was performed from the immunoprecipitate using PSMB2 antibody (CAB5483) at a dilution of 1:1000.
