The PSMB9/LMP2 Antibody (CAB1771) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets the PSMB9 protein, also known as the immunoproteasome subunit beta-1i. It is highly reactive with human samples and has been validated for use in Western blot applications.The PSMB9 protein is a critical component of the immunoproteasome, which plays a key role in antigen processing and presentation. By targeting PSMB9, researchers can investigate its functions in various cell types and its involvement in immune responses.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PSMB9/LMP2 Antibody
SKU:
CAB1771
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LMP2, PRAAS3, PSMB6i, RING12, beta1i, PSMB9/LMP2
Positive Sample:
Raji, THP-1
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
23kDa
Observed MW:
23kDa
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.
Purification Method
Affinity purification
Gene ID
5698
RRID
AB_2763813
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PSMB9/LMP2 Rabbit pAb (CAB1771) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using PSMB9/LMP2 Rabbit pAb (CAB1771).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
