The PSMD4 Monoclonal Antibody (CAB3663) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, displays high reactivity with human samples and is validated for use in various applications, including Western blotting.PSMD4 is essential for the proper functioning of the proteasome complex, which plays a crucial role in protein degradation and maintaining cellular homeostasis. Dysregulation of the proteasome complex, including PSMD4, has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PSMD4 Monoclonal Antibody
SKU:
CAB3663
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0818
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5, PSMD4
Positive Sample:
A549, U-251MG, Mouse testis, Rat brain
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
55kDa
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.
Purification Method
Affinity purification
Gene ID
5710
RRID
AB_2863108
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PSMD4 Rabbit mAb (CAB3663) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of NIH-3T3 cells using PSMD4 Rabbit mAb (CAB3663) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
