The PTCD3 Antibody (CAB8497) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards PTCD3 in human samples, making it a reliable choice for Western blot applications. By binding to PTCD3, this antibody allows for the detection and analysis of the protein in various cell types, facilitating research into mitochondrial gene expression and RNA metabolism.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PTCD3 Antibody
SKU:
CAB8497
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COXPD51, MRP-S39, PTCD3
Positive Sample:
A-549, SGC-7901, LO2, K-562
Cellular Localization:
Mitochondrion.
Calculated MW:
79kDa
Observed MW:
79kDa
Enables rRNA binding activity and ribosomal small subunit binding activity. Involved in mitochondrial translation. Located in several cellular components, including cytosol; mitochondrion; and nucleoplasm. Implicated in combined oxidative phosphorylation deficiency 51.
Purification Method
Affinity purification
Gene ID
55037
RRID
AB_2771865
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PTCD3 Rabbit pAb (CAB8497) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using PTCD3 Rabbit pAb (CAB8497) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
