The PTPRB Polyclonal Antibody (CAB6957) is a versatile tool for researchers studying PTPRB, a protein tyrosine phosphatase involved in various cellular processes such as cell adhesion and signaling. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications.PTPRB, also known as VE-PTP, plays a crucial role in vascular development and maintenance by regulating endothelial cell junctions and vascular permeability. Dysregulation of PTPRB has been implicated in various diseases including cancer and vascular disorders, highlighting its potential as a therapeutic target.
By targeting the PTPRB protein, this antibody enables precise detection and analysis of PTPRB expression in different cell types, offering valuable insights into its functions and potential roles in disease pathogenesis. Its specificity and reliability make it an essential tool for research in vascular biology, cancer, and other related fields.
Product Name:
PTPRB Polyclonal Antibody
SKU:
CAB6957
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Protein corresponding to a sequence within amino acids 1173-1260 of human PTPRB (NP_002828.3).
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and one intracytoplasmic catalytic domain, thus belongs to receptor type PTP. The extracellular region of this PTP is composed of multiple fibronectin type_III repeats, which was shown to interact with neuronal receptor and cell adhesion molecules, such as contactin and tenascin C. This protein was also found to interact with sodium channels, and thus may regulate sodium channels by altering tyrosine phosphorylation status. The functions of the interaction partners of this protein implicate the roles of this PTP in cell adhesion, neurite growth, and neuronal differentiation. Alternate transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5787
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251 MG cells using PTPRB Rabbit pAb(CAB6957) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
