The Nectin 2/CD112 Antibody (CAB5378) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RRID AB_2766188 Gene ID 5819 Swiss Prot Synonym HVEB; PRR2; CD112; PVRL2; PVRR2; Nectin 2/CD112
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Nectin 2/CD112 Antibody
SKU:
CAB5378
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HVEB, PRR2, CD112, PVRL2, PVRR2, Nectin 2/CD112
Positive Sample:
MCF-7, SW620, A-549, SKOV3
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
58kDa
Observed MW:
72kDa
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RRID AB_2766188 Gene ID 5819 Swiss Prot Synonym HVEB; PRR2; CD112; PVRL2; PVRR2; Nectin 2/CD112
Purification Method:
Affinity purification
Gene ID:
5819
RRID:
AB_2766188
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Nectin 2/CD112 Rabbit pAb (CAB5378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
