The PDXK Antibody (CAB6687) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the pyridoxal kinase protein, researchers can detect and analyze its expression in various cell types, offering valuable insights into its role in cellular processes related to vitamin B6 metabolism.Pyridoxal kinase is crucial for converting pyridoxal (a form of vitamin B6) into its active form, pyridoxal 5'-phosphate, which is involved in a wide range of biochemical reactions in the body.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PDXK Antibody
SKU:
CAB6687
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HeLa, U-251MG, Molt-4, Mouse kidney, Mouse liver, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Purification Method
Affinity purification
Gene ID
8566
RRID
AB_2767271
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PDXK Rabbit pAb (CAB6687) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
