The QuickStep Human IgA (Immunoglobulin A) ELISA Kit is a highly reliable and sensitive tool for the precise measurement of IgA levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional accuracy and reproducibility, making it an ideal choice for a variety of research applications.IgA is a crucial antibody that plays a key role in the body's immune response, particularly in mucosal immunity.
Abnormal levels of IgA have been associated with a wide range of autoimmune diseases, allergies, and infections, making it a valuable biomarker for studying these conditions and developing potential treatments.With its high sensitivity and specificity, the QuickStep Human IgA ELISA Kit provides researchers with a powerful tool for investigating the role of IgA in various diseases and developing new therapeutic strategies.
Product Name:
QuickStep Human IgA ELISA Kit
Product Code:
QSES023
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
0.14 ng/mL
Range:
0.78-50 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
IgA
Application:
This ELISA kit applies to the in vitro quantitative determination of IgA concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes IgA in samples. No significant cross-reactivity or interference between IgA and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 200000-800000 fold, saliva samples at 5000-10000 fold and urine samples at 20-100 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgA are added to the micro ELISA plate wells. Human IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgA. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
2.35
4.49
21.37
2.45
4.10
20.27
Standard deviation
0.15
0.22
0.91
0.14
0.23
1.11
CV (%)
6.41
4.95
4.25
5.69
5.66
5.48
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
97-110
102
EDTA plasma(n=8)
88-98
93
Urine(n=8)
96
Samples were spiked with high concentrations of Human IgA and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
