Description
Human TG (Thyroglobulin) Quickstep ELISA Kit
The QuickStep Human TG (Thyroglobulin) ELISA Kit is a specialized assay designed for the quantitative measurement of Thyroglobulin levels in human biological samples. Thyroglobulin, abbreviated as TG, is a glycoprotein that serves as a precursor in the synthesis of thyroid hormones within the thyroid gland.
It plays a crucial role in the production, storage, and secretion of thyroid hormones, making it an essential marker in thyroid function assessment. This high-sensitivity ELISA kit is tailored to provide precise and accurate quantification of Thyroglobulin, offering valuable insights into thyroid health, thyroid disorders, and thyroid cancer. Thyroglobulin is a critical biomarker used in the diagnosis, surveillance, and management of various thyroid conditions and pathologies. Manufactured with a focus on performance and reliability, the QuickStep Human TG ELISA Kit ensures exceptional sensitivity and specificity, delivering reproducible results for research studies, clinical investigations, and diagnostic applications. Researchers and healthcare professionals can rely on this kit to generate robust data for understanding thyroid function and thyroid-related disorders, making it an excellent choice for thyroid research and clinical assessments.
| Product Name: | Human TG (Thyroglobulin) Quickstep ELISA Kit |
| Product Code: | AEES00645 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 2.5h |
| Reactivity: | Human |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 3.38 pg/mL |
| Sample Type & Sample Volume: | Serum and plasma, 50?L |
| Specificity: | This kit recognizes Human TG in samples. No significant or interference between Human TG and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Samples (or Standards) and biotinylated detection antibody specific for Human TG are added to the micro ELISA plate wells. Human TG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for six months. After test, the unused wells and reagents should be stored according to the table below.
|
| 1. | Add 50?L standard or sample to the wells, immediately add 50?L Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50?L Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
Thyroglobulin (Tg) is a large glycoprotein that is stored in follicles in healthy thyroid tissue as colloid where it acts as a substrate for thyroid hormones synthesis. Thyroglobulin is a homologous dimer with a molecular weight of about 660 kDa. Human protein consists of 2,767 amino acid residues and is the storage form of iodine in the thyroid gland, precursor of the iodinated thyroid hormones, thyroxine (T4) and triiodothyronine (T3). It is exclusively produced by normal or well-differentiated malignant thyrocytes. Then, its tissue-specific origin makes it eminently suitable as a serum tumor indicator in patients followed-up for differentiated thyroid carcinoma (DTC) after thyroid removal and remnants ablation[1].The most studied polymorphisms in the TG gene are rs2069550 (E10SNP158), rs853326 (E12SNP), and rs180223 (E10SNP24) in exons 10–12 and rs2076740 (E33SNP) in exon 33 SNP, which were reported to be associated with AITD[2].
