The QuickStep Sheep E2 (Estradiol) ELISA Kit is specifically designed for the accurate detection of estradiol levels in sheep serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it perfect for a variety of research applications.Estradiol is a key hormone in sheep reproduction, playing a crucial role in the estrous cycle and fertility.
Monitoring estradiol levels is essential for understanding and managing reproductive health in sheep, making this kit an invaluable tool for researchers and veterinarians alike.With the QuickStep Sheep E2 (Estradiol) ELISA Kit, you can confidently study and monitor estradiol levels in sheep, leading to advancements in sheep reproductive health and productivity.
Product Name:
QuickStep Sheep Estradiol ELISA Kit
Product Code:
QSES058
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Sheep
Sensitivity:
12.74 pg/mL
Range:
31.25-2000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Sheep serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
105.43
221.46
951.12
100.06
221.74
987.56
Standard deviation
6.37
11.25
46.13
5.58
9.45
44.14
CV (%)
6.04
5.08
4.85
5.58
4.26
4.47
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-102
94
EDTA plasma(n=8)
92-108
99
Samples were spiked with high concentrations of Sheep E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
