The RAB24 Antibody (CAB8394) is a high-quality antibody developed for reliable detection and analysis of target proteins. RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking (Olkkonen et al., 1993 [PubMed 8126105]).
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAB24 Antibody
SKU:
CAB8394
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAB24
Positive Sample:
HT-1080, MCF7, SH-SY5Y, 293T, Mouse brain
Cellular Localization:
Cytoplasm, Lipid-Anchor, Membrane, Cytosol.
Calculated MW:
23kDa
Observed MW:
23kDa
RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking (Olkkonen et al., 1993 [PubMed 8126105]).
Purification Method
Affinity purification
Gene ID
53917
RRID
AB_2771905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RAB24 Rabbit pAb (CAB8394) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg RAB24 antibody (CAB8394). Western blot was performed from the immunoprecipitate using RAB24 antibody (CAB8394) at a dilution of 1:1000.