The RAB5C Polyclonal Antibody (CAB21583) is a high-quality antibody developed for reliable detection and analysis of target proteins. Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RAB5C Polyclonal Antibody
SKU:
CAB21583
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RABL, L1880, RAB5L, RAB5CL, RAB5C
Positive Sample:
A549
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Early Endosome Membrane, Lipid-Anchor, Melanosome.
Calculated MW:
23kDa
Observed MW:
23kDa
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
Purification Method
Affinity purification
Gene ID
5878
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A549 cells using RAB5C Rabbit pAb (CAB21583) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
