The Rabbit Anti-Chicken IgY (H+L) (CABS082) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards chicken IgY and is suitable for various applications, including ELISA, immunohistochemistry, and immunofluorescence.Chicken IgY is a key component of the avian immune system, playing a crucial role in defense against pathogens and maintaining immune homeostasis. Studying the properties and functions of chicken IgY is essential for understanding avian immune responses and developing novel biotechnological applications, such as in antibody production and diagnostics.
This antibody is validated for use in WB applications and has demonstrated reactivity against Chicken samples.
Product Name:
Rabbit Anti-Chicken IgY (H+L)
SKU:
CABS082
Size:
100μL
Reactivity:
Chicken
Conjugate:
Unconjugated
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:500 - 1:1000
Positive Sample:
Rabbit Anti-Chicken IgY
Observed MW:
25kDa/67-70kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Chicken IgY, using Rabbit Anti-Chicken IgY (H+L) (CABS082) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
