The Rabbit Anti-Goat IgG (H+L) (CABS081) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with Goat IgG and is validated for use in various applications, including Western blotting.Goat IgG is an important component of immune responses and is often used as a marker in immunoassays and other experimental procedures. This antibody binds specifically to Goat IgG, enabling accurate detection and analysis in a wide range of experimental settings.Ideal for researchers in immunology, antibody development, and related fields, the Rabbit Anti-Goat IgG Antibody provides reliable and consistent results, making it a valuable tool for studying immune responses and antibody interactions.
This antibody is validated for use in WB applications and has demonstrated reactivity against Goat samples.
Product Name:
Rabbit Anti-Goat IgG (H+L)
SKU:
CABS081
Size:
100μL
Reactivity:
Goat
Conjugate:
Unconjugated
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:500 - 1:1000
Positive Sample:
Goat IgG
Observed MW:
25kDa(Lightchain),55kDa(Heavychain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Goat IgG using Rabbit Anti-Goat IgG (H+L) (CABS081) at 1:1000 dilution. Lysates/proteins: 5 ng - 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
