The Rabbit anti V5-Tag pAb (CABE071) is a high-quality antibody developed for reliable detection and analysis of target proteins. Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
This antibody is validated for use in WB applications and has demonstrated reactivity against Species independent samples.
Product Name:
Rabbit anti V5-Tag pAb
SKU:
CABE071
Size:
100μL
Reactivity:
Species independent
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:1000 - 1:2000
Synonyms:
V5, V5 tag, V5-tag
Positive Sample:
293T
Observed MW:
Refer to Figures
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2863796
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein,using Rabbit anti V5-Tag pAb (CABE071) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
