The Rabbit bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit is a specialized assay designed for the quantitative detection of basic fibroblast growth factor (bFGF or FGF2) levels in various rabbit biological samples. Basic fibroblast growth factor is a key regulator of cell growth, proliferation, and differentiation, playing a crucial role in tissue development, wound healing, and angiogenesis. Accurate measurement of bFGF levels is essential for studying cellular processes, tissue repair mechanisms, and pathological conditions related to growth factor dysregulation.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in the quantitative assessment of bFGF levels. Manufactured under strict quality control standards, the kit demonstrates robust performance and user-friendly protocols, making it an invaluable tool for researchers in the fields of cell biology, regenerative medicine, and developmental biology.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit bFGF/FGF2. You can calculate the concentration of Rabbit bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
88-103
91-105
Average (%)
102
95
98
1:4
Range (%)
97-111
89-102
90-104
Average (%)
104
94
95
1:8
Range (%)
97-113
90-104
89-102
Average (%)
103
95
96
1:16
Range (%)
91-107
90-106
91-106
Average (%)
98
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.19
154.81
340.85
48.38
139.46
315.58
Standard deviation
3.26
6.89
16.19
2.5
7.25
12.21
C V (%)
6.63
4.45
4.75
5.17
5.2
3.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit bFGF/FGF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit bFGF/FGF2 in samples. No significant cross-reactivity or interference between Rabbit bFGF/FGF2 and analogues was observed.
