The Rabbit IL-10 ELISA Kit is a highly specialized assay designed to quantitatively measure the levels of Interleukin-10 (IL-10) in various rabbit biological samples.
IL-10 is a crucial anti-inflammatory cytokine that plays a key role in regulating immune responses, maintaining immune homeostasis, and modulating inflammatory processes. Accurate detection and quantification of IL-10 levels are essential for evaluating immune modulation, inflammatory responses, and the development of potential therapeutic strategies targeting immune-related disorders. The Rabbit IL-10 ELISA Kit provides researchers with a precise and reliable tool to explore the role of IL-10 in rabbit physiology and pathophysiology. With exceptional sensitivity and specificity, this ELISA kit delivers consistent and reproducible results, enabling researchers to gain deeper insights into the complex interplay of IL-10 in immune regulation. Manufactured under stringent quality controls, this kit ensures robust performance and ease of use, making it an indispensable resource for cutting-edge research in immunology and beyond.
Product Name:
Rabbit IL-10 ELISA Kit
SKU:
AEES00570
Target:
Rabbit IL-10
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-10. You can calculate the concentration of Rabbit IL-10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
94-108
96-112
Average (%)
102
99
102
1:4
Range (%)
88-100
81-91
83-99
Average (%)
94
86
90
1:8
Range (%)
90-103
81-93
87-102
Average (%)
97
86
94
1:16
Range (%)
93-107
81-90
85-98
Average (%)
99
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.86
106.52
337.47
52.51
102.92
351.65
Standard deviation
2.6
5.54
10.63
3.18
4.63
16.6
C V (%)
5.21
5.2
3.15
6.06
4.5
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IL-10 in samples. No significant cross-reactivity or interference between Rabbit IL-10 and analogues was observed.
