SynonymsCD62E,CD62-E,E-Selectin,ELAM,ELAM1,ESEL,SEL-E,E-LAM,E-LAM1,E-SEL,LECAM2,Endothelial Leukocyte Adhesion Molecule 1,CD62 Antigen-Like Family Member E
ReactivityRabbit
Sample TypeSerum, Plasma And Other Biological Fluids
SKU:
RBDL00068
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27113
Sensitivity:
2.27ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62E,CD62-E,E-Selectin,ELAM,ELAM1,ESEL,SEL-E,E-LAM,E-LAM1,E-SEL,LECAM2,Endothelial Leukocyte Adhesion Molecule 1,CD62 Antigen-Like Family Member E
The Rabbit Selectin Endothelium (SELE) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of SELE levels in rabbit serum, plasma, and cell culture supernatants. Selectin Endothelium (SELE) is a key protein involved in the adhesion of leukocytes to endothelial cells, playing a crucial role in inflammatory responses and immune system regulation. Dysregulation of SELE has been linked to various inflammatory disorders and diseases, making it an important biomarker for research and clinical studies.
With its reliable and reproducible results, this ELISA kit is ideal for a wide range of applications, from basic research to drug development and biomarker discovery. Trust in the Rabbit Selectin Endothelium (SELE) ELISA Kit for accurate and precise measurements of SELE levels in rabbit samples.
Product Name:
Rabbit Selectin, Endothelium (SELE) ELISA Kit
Product Code:
RBDL00068
Size:
96 Assays
Target:
SELE
Synonyms:
CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
