The RABL3 Antibody (CAB14327) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting RABL3 in human samples and is validated for use in Western blot applications. By targeting the RABL3 protein, this antibody allows for the precise detection and analysis of RABL3 expression in various cell types, making it an excellent choice for studies in cell biology and cancer research.RABL3 plays a crucial role in regulating intracellular vesicle trafficking and membrane dynamics, making it a key player in cellular processes such as endocytosis and membrane recycling.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RABL3 Antibody
SKU:
CAB14327
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PNCA5, RABL3
Positive Sample:
MCF7, Mouse brain, Mouse lung, Mouse testis
Calculated MW:
26kDa
Observed MW:
26kDa
Predicted to enable GTP binding activity; GTPase activity; and protein homodimerization activity. Involved in regulation of Ras protein signal transduction and regulation of protein lipidation. Predicted to be active in endomembrane system. Implicated in pancreatic cancer.
Purification Method
Affinity purification
Gene ID
285282
RRID
AB_2761192
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RABL3 Rabbit pAb (CAB14327) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using RABL3 Rabbit pAb (CAB14327) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
