The Rac1/2/3 Monoclonal Antibody (CAB5080) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for experiments involving Western blot analysis.RAC proteins are essential players in multiple cellular processes, including cell adhesion, motility, and invasion, making them attractive targets for investigation in cancer biology and cell biology research. The RAC1/2/3 Rabbit Monoclonal Antibody enables accurate detection and quantification of RAC1, RAC2, and RAC3 proteins in various cell types, providing insights into their roles in signaling pathways and cellular functions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rac1/2/3 Monoclonal Antibody
SKU:
CAB5080
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1165
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5879 5880 5881
RRID
AB_2863437
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rac1/2/3 Rabbit mAb (CAB5080) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Rac1/2/3 Rabbit mAb (CAB5080) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Rac1/2/3 Rabbit mAb (CAB5080) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.