The Rat Aβ1-42 (Amyloid Beta 1-42) ELISA Kit is specifically designed for the precise detection of amyloid beta 1-42 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it well-suited for a variety of research applications.Amyloid beta 1-42 is a key protein involved in the pathogenesis of Alzheimer's disease, playing a crucial role in the formation of amyloid plaques in the brain.
By measuring levels of amyloid beta 1-42, researchers can gain valuable insights into the progression of Alzheimer's disease and potentially identify new therapeutic targets.Overall, the Rat Aβ1-42 (Amyloid Beta 1-42) ELISA Kit is an essential tool for studying neurodegenerative disorders and advancing our understanding of Alzheimer's disease. Its reliability and precision make it a valuable asset for researchers in the field.
Product Name:
Rat A beta1-42 (Amyloid Beta 1-42) ELISA Kit
SKU:
RTES00978
Target:
Rat A beta1-42 (Amyloid Beta 1-42)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Aβ1-42. You can calculate the concentration of Rat Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
86-97
93-105
Average (%)
103
92
98
1:4
Range (%)
99-115
83-96
93-108
Average (%)
106
88
99
1:8
Range (%)
99-113
80-93
92-105
Average (%)
105
87
98
1:16
Range (%)
93-107
83-94
92-105
Average (%)
100
88
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
101
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.8
84.8
397.7
52.0
93.2
360.7
Standard deviation
3
4.2
12.7
3.4
4.9
15.5
C V (%)
6.15
4.95
3.19
6.54
5.26
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Aβ1-42 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Aβ1-42 in samples. No significant cross-reactivity or interference between Rat Aβ1-42 and analogues was observed.
