The Rat ACV-A (Activin A) CLIA Kit is a cutting-edge assay designed for the sensitive and specific detection of Activin A levels in rat biological samples, including serum, plasma, and tissue extracts. This kit offers high accuracy and reproducibility, making it an invaluable tool for researchers in various fields.Activin A is a key protein involved in various physiological processes, including inflammation, tissue repair, and embryonic development. Dysregulation of Activin A levels has been linked to a range of diseases such as cancer, inflammatory disorders, and metabolic conditions, highlighting the importance of monitoring its expression in research studies.
With its advanced technology and reliable performance, the Rat ACV-A CLIA Kit empowers scientists to explore the role of Activin A in different biological systems, paving the way for new insights and potential therapeutic interventions.
Product Name:
Rat ACV-A (Activin A) CLIA Kit
SKU:
RTES00001
Target:
Rat ACV-A (Activin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ACV-A. You can calculate the concentration of Rat ACV-A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
92-105
93-107
Average (%)
109
98
99
1:4
Range (%)
88-102
91-104
91-103
Average (%)
95
96
98
1:8
Range (%)
93-106
91-104
94-107
Average (%)
98
99
101
1:16
Range (%)
88-102
83-98
99-113
Average (%)
95
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.53
110.35
495.43
53.43
106.88
489.63
Standard deviation
5.14
11.61
42.56
5.64
12.29
55.52
C V (%)
9.26
10.52
8.59
10.56
11.5
11.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ACV-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ACV-A in samples. No significant cross-reactivity or interference between Rat ACV-A and analogues was observed.
