Rat ADAMTS13/VWFCP (Von Willebrand Factor Cleaving Protease) CLIA Kit
The Rat ADAMTS13 (VWFCP) Von Willebrand Factor Cleaving Protease CLIA Kit is a state-of-the-art assay kit designed for the accurate quantification of ADAMTS13 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.ADAMTS13 is a key enzyme responsible for cleaving von Willebrand factor (VWF), playing a critical role in regulating hemostasis and thrombosis. Dysregulation of ADAMTS13 has been implicated in various diseases, including thrombotic thrombocytopenic purpura (TTP) and other thrombotic disorders.
Therefore, accurate measurement of ADAMTS13 levels is essential for understanding these conditions and developing targeted therapies.With its advanced technology and superior performance, the Rat ADAMTS13 (VWFCP) Von Willebrand Factor Cleaving Protease CLIA Kit is a valuable tool for researchers studying hemostasis, thrombosis, and related diseases in rat models.
Product Name:
Rat ADAMTS13/VWFCP (Von Willebrand Factor Cleaving Protease) CLIA Kit
SKU:
RTES00595
Target:
Rat ADAMTS13/VWFCP (Von Willebrand Factor Cleaving Protease)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ADAMTS13/VWFCP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADAMTS13/VWFCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADAMTS13/VWFCP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ADAMTS13/VWFCP. You can calculate the concentration of Rat ADAMTS13/VWFCP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
96-112
91-102
Average (%)
100
103
96
1:4
Range (%)
88-100
87-100
95-111
Average (%)
94
92
101
1:8
Range (%)
86-98
88-101
100-114
Average (%)
91
96
107
1:16
Range (%)
89-103
91-108
84-99
Average (%)
94
98
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.51
143.28
345.04
54.95
146.76
335.03
Standard deviation
5.89
14.39
38.13
5.15
15.85
23.62
C V (%)
10.61
10.04
11.05
9.37
10.8
7.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ADAMTS13/VWFCP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ADAMTS13/VWFCP in samples. No significant cross-reactivity or interference between Rat ADAMTS13/VWFCP and analogues was observed.
