The Rat Adiponectin Receptor 2 (AdipoR2) ELISA Kit is a powerful tool for precise measurement of AdipoR2 levels in rat biological samples, including serum, plasma, and tissue lysates. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for various research studies. AdipoR2 is a key receptor involved in adiponectin signaling, playing a critical role in metabolic regulation, glucose homeostasis, and insulin sensitivity.
Dysregulation of AdipoR2 has been linked to metabolic disorders such as obesity, diabetes, and cardiovascular diseases, making it a valuable target for therapeutic interventions and drug development. This ELISA kit enables researchers to study the expression and function of AdipoR2 in different physiological and pathological conditions, paving the way for novel insights and potential treatment strategies.
Product Name:
Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit
Product Code:
RTDL00030
Size:
96 Assays
Target:
ADIPOR2
Synonyms:
PAQR2, ACDCR2, Progestin and AdipoQ Receptor Family Member II
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
