The Rat ADP-Acrp30 (Adiponectin) CLIA Kit is specifically designed for the accurate quantification of adiponectin levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Adiponectin is a key adipokine that plays a critical role in regulating metabolism and insulin sensitivity. It is involved in various physiological processes, including inflammation, lipid metabolism, and insulin signaling.
Dysregulation of adiponectin levels is associated with metabolic disorders, cardiovascular diseases, and obesity, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.This CLIA kit provides researchers with a reliable tool to study the role of adiponectin in health and disease, offering accurate and reproducible results that can enhance our understanding of adiponectin biology and potential therapeutic strategies.
Product Name:
Rat ADP/Acrp30 (Adiponectin) CLIA Kit
SKU:
RTES00177
Target:
Rat ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ADP/Acrp30. You can calculate the concentration of Rat ADP/Acrp30 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
89-104
89-102
Average (%)
95
97
96
1:4
Range (%)
102-116
97-112
86-98
Average (%)
109
103
91
1:8
Range (%)
99-113
96-113
86-98
Average (%)
106
103
91
1:16
Range (%)
94-109
92-106
94-109
Average (%)
100
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
1.98
7.59
1.09
2.06
8.18
Standard deviation
0.11
0.23
0.51
0.14
0.15
0.51
C V (%)
10.48
11.62
6.72
12.84
7.28
6.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ADP/Acrp30 in samples. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed.
