Rat AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
The RatAGER Advanced Glycosylation End Product Specific Receptor CLIA Kit is specifically designed for the accurate detection of AGEs (advanced glycosylation end products) in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying AGE-related diseases such as diabetes, cardiovascular diseases, and neurodegenerative disorders.
AGEs are formed through a non-enzymatic reaction between sugars and proteins, leading to protein cross-linking and altered cellular functions. The RatAGER CLIA Kit provides a valuable tool for researchers investigating the role of AGEs in aging and age-related diseases, offering a comprehensive solution for detecting and quantifying these important biomarkers.
Product Name:
Rat AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
SKU:
RTES00398
Target:
Rat AGER (Advanced Glycosylation End Product Specific Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AGER. You can calculate the concentration of Rat AGER in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
97-113
101-118
Average (%)
107
103
109
1:4
Range (%)
95-112
95-107
91-105
Average (%)
102
101
96
1:8
Range (%)
85-97
101-115
84-99
Average (%)
92
108
90
1:16
Range (%)
97-107
91-108
97-110
Average (%)
102
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
86-100
94
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.3
122.88
267.75
39.89
130.59
287.0
Standard deviation
4.83
13.82
19.49
4.23
10.77
23.82
C V (%)
11.69
11.25
7.28
10.6
8.25
8.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AGER concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AGER in samples. No significant cross-reactivity or interference between Rat AGER and analogues was observed.
