Description
Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
The Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit is a specialized assay developed for the quantitative detection of AGER levels in various rat biological samples.
AGER, also known as RAGE (Receptor for Advanced Glycation End Products), is a multi-ligand receptor involved in diverse cellular processes, including inflammation, immune response, and cell proliferation. AGER plays a crucial role in initiating cellular signaling pathways in response to advanced glycation end products, which contribute to various pathological conditions. Accurate measurement of AGER is essential for understanding its role in diabetic complications, neurodegenerative diseases, cancer, and cardiovascular disorders. The Rat AGER ELISA Kit enables researchers to study the expression and function of AGER in rat models, providing insights into the mechanisms underlying disease pathogenesis and the development of potential therapeutic strategies. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, the kit provides reliable performance and user-friendly protocols, making it an excellent choice for research aimed at elucidating the functions of AGER and its implications in various diseases.
| Product Name: | Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit |
| Product Code: | AEES00489 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat AGER in samples. No significant cross-reactivity or interference between Rat AGER and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat AGER concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AGER. You can calculate the concentration of Rat AGER in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
