The Rat AGRN (Agrin) ELISA Kit is specifically designed for precise and reliable detection of agrin levels in rat serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results that are essential for a variety of research applications.Agrin is a key protein that plays a critical role in synaptic plasticity and development, making it a valuable biomarker for studying neurological disorders, neuromuscular diseases, and other conditions affecting the nervous system.
By accurately measuring agrin levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic opportunities.With its robust performance and easy-to-use format, the Rat AGRN (Agrin) ELISA Kit is a valuable tool for researchers looking to study agrin and its role in various biological processes. Get reliable and precise results with this high-quality ELISA kit from Assay Genie.
Product Name:
Rat AGRN (Agrin) ELISA Kit
SKU:
RTES01016
Target:
Rat AGRN (Agrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AGRN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGRN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AGRN. You can calculate the concentration of Rat AGRN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
91-104
93-108
Average (%)
91
98
100
1:4
Range (%)
86-100
82-94
83-97
Average (%)
93
87
90
1:8
Range (%)
87-101
86-97
83-94
Average (%)
95
91
89
1:16
Range (%)
94-106
84-99
87-101
Average (%)
100
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.05
257.27
686.98
110.86
277.63
701.4
Standard deviation
6.54
10.63
31.19
7.74
14.85
24.27
C V (%)
6.29
4.13
4.54
6.98
5.35
3.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat AGRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AGRN in samples. No significant cross-reactivity or interference between Rat AGRN and analogues was observed.
