The Rat AGT (Angiotensinogen) CLIA Kit is specifically designed for the precise measurement of angiotensinogen levels in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing consistent and accurate results that are suitable for a variety of research purposes.Angiotensinogen is a key component in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of angiotensinogen has been linked to various conditions such as hypertension, cardiovascular diseases, and kidney disorders, making it a valuable biomarker for studying and developing therapies for these conditions.
Overall, the Rat AGT CLIA Kit offers researchers a reliable and efficient tool for investigating the role of angiotensinogen in disease progression and treatment, ultimately contributing to advancements in the field of molecular biology and healthcare.
Product Name:
Rat AGT (Angiotensinogen) CLIA Kit
SKU:
RTES00116
Target:
Rat AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AGT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AGT. You can calculate the concentration of Rat AGT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
102-120
99-117
Average (%)
103
109
107
1:4
Range (%)
91-109
94-107
97-109
Average (%)
99
100
103
1:8
Range (%)
94-105
93-107
101-119
Average (%)
99
100
109
1:16
Range (%)
100-114
101-118
85-98
Average (%)
108
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.93
78.0
189.83
25.89
75.88
189.94
Standard deviation
3.39
7.53
21.49
2.75
8.97
16.22
C V (%)
12.59
9.65
11.32
10.62
11.82
8.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AGT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AGT in samples. No significant cross-reactivity or interference between Rat AGT and analogues was observed.
