Rat Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
The Rat Alkaline Sphingomyelinase (ALK-SMase) ELISA Kit is a highly reliable and accurate tool for detecting levels of alkaline sphingomyelinase in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring that you obtain precise and reproducible results for your research needs.Alkaline sphingomyelinase plays a key role in sphingolipid metabolism and has been implicated in various physiological processes and diseases. By accurately measuring alkaline sphingomyelinase levels, researchers can gain valuable insights into its functions and potential implications in conditions such as cancer, inflammation, and metabolic disorders.
With its user-friendly design and robust performance, the Rat ALK-SMase ELISA Kit is an essential tool for studying alkaline sphingomyelinase and its impact on health and disease. It is an indispensable asset for researchers seeking to unravel the complexities of sphingolipid biology and develop novel therapeutic strategies.
Product Name:
Rat Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
91-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
