Rat alpha1-AGP (alpha1-Acid Glycoprotein) CLIA Kit
The Rat Alpha1-AGP (Alpha1-Acid Glycoprotein) CLIA Kit is a cutting-edge assay designed for the accurate measurement of alpha1-AGP levels in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Alpha1-AGP is a critical protein involved in various physiological processes, including inflammation, drug metabolism, and immune response regulation.
Elevated levels of alpha1-AGP have been linked to conditions such as liver disease, cancer, and infectious diseases, making it a valuable biomarker for disease research and therapeutic development.With its advanced technology and user-friendly protocols, the Rat Alpha1-AGP CLIA Kit is a valuable tool for researchers studying the role of alpha1-AGP in health and disease. Order yours today and unlock new insights into the complex mechanisms of alpha1-AGP biology.
Product Name:
Rat alpha1-AGP (alpha1-Acid Glycoprotein) CLIA Kit
SKU:
RTES00597
Target:
Rat alpha1-AGP (alpha1-Acid Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat α1-AGP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat α1-AGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat α1-AGP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat α1-AGP. You can calculate the concentration of Rat α1-AGP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
99-115
96-108
Average (%)
91
106
103
1:4
Range (%)
91-103
87-100
97-113
Average (%)
97
94
105
1:8
Range (%)
93-108
86-97
90-104
Average (%)
98
91
95
1:16
Range (%)
94-112
88-101
84-100
Average (%)
102
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
99-116
106
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.96
6.75
35.72
4.35
6.56
32.85
Standard deviation
0.39
0.8
3.39
0.41
0.54
2.1
C V (%)
9.85
11.85
9.49
9.43
8.23
6.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat α1-AGP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat α1-AGP in samples. No significant cross-reactivity or interference between Rat α1-AGP and analogues was observed.
