The Rat ANG II (Angiotensin II) ELISA Kit is specially designed for the accurate measurement of angiotensin II levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Angiotensin II is a key hormone involved in the regulation of blood pressure and fluid balance, playing a crucial role in the renin-angiotensin system. Dysregulation of angiotensin II levels has been linked to various cardiovascular diseases, renal disorders, and hypertension, making it a valuable biomarker for understanding these conditions and potential therapeutic interventions.
With its reliable performance and ease of use, the Rat ANG II ELISA Kit is an essential tool for researchers studying the physiological and pathological roles of angiotensin II in animal models. Get accurate and reliable data with this advanced assay kit from Assay Genie.
Product Name:
Rat ANG II (Angiotensin II) ELISA Kit
SKU:
RTES01000
Target:
Rat ANG II (Angiotensin II)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
92-107
94-107
Average (%)
90
98
100
1:4
Range (%)
84-95
91-103
96-108
Average (%)
90
97
102
1:8
Range (%)
86-97
90-102
98-113
Average (%)
92
96
104
1:16
Range (%)
89-100
87-101
93-108
Average (%)
94
94
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.8
133.7
360.1
46.1
133.4
382.0
Standard deviation
3
7.6
13.0
3.2
7.1
15.7
C V (%)
6.41
5.68
3.61
6.94
5.32
4.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang-â…¡ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang-â…¡ in samples. No significant cross-reactivity or interference between Ang-â…¡ and analogues was observed.
