The Rat ANG1 (Angiopoietin-1) CLIA Kit is a highly accurate assay designed for the quantitative detection of Angiopoietin-1 levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiopoietin-1 is a key factor in angiogenesis, playing a vital role in the regulation of blood vessel formation and vascular stability. Dysregulation of Angiopoietin-1 has been implicated in various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for investigating these pathological processes and developing potential therapeutic interventions.
With its advanced technology and unparalleled performance, the Rat ANG1 CLIA Kit is an essential tool for researchers studying angiogenesis, vascular biology, and related fields. Get accurate and reproducible results with this innovative assay for comprehensive analysis of Angiopoietin-1 levels in rat samples.
Product Name:
Rat ANG1 (Angiopoietin 1) CLIA Kit
SKU:
RTES00389
Target:
Rat ANG1 (Angiopoietin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ANG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ANG1. You can calculate the concentration of Rat ANG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
96-108
89-104
Average (%)
93
102
97
1:4
Range (%)
96-113
99-116
99-114
Average (%)
104
107
106
1:8
Range (%)
97-110
84-97
89-101
Average (%)
103
91
96
1:16
Range (%)
92-106
97-114
93-108
Average (%)
98
104
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.92
217.65
825.83
113.52
214.1
799.95
Standard deviation
11.41
21.74
56.73
11.75
17.56
66.96
C V (%)
10.38
9.99
6.87
10.35
8.2
8.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ANG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ANG1 in samples. No significant cross-reactivity or interference between Rat ANG1 and analogues was observed.
