Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit
The Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit is specifically designed for the precise quantification of ANGPTL3 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ANGPTL3 is a key regulator of lipid metabolism and has been implicated in various metabolic disorders such as dyslipidemia, obesity, and cardiovascular diseases.
Its role in modulating lipid levels and insulin sensitivity makes it a valuable biomarker for studying metabolic diseases and evaluating potential therapeutic interventions.Overall, the Rat ANGPTL3 ELISA Kit provides researchers with a reliable tool for investigating the role of ANGPTL3 in metabolic pathways and exploring its potential as a target for therapeutic interventions.
Product Name:
Rat ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit
SKU:
RTES01074
Target:
Rat ANGPTL3 (Angiopoietin Like Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ANGPTL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANGPTL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ANGPTL3. You can calculate the concentration of Rat ANGPTL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
86-101
89-101
Average (%)
94
92
96
1:4
Range (%)
88-102
82-92
84-94
Average (%)
95
86
89
1:8
Range (%)
90-105
88-100
86-100
Average (%)
97
93
93
1:16
Range (%)
88-99
83-96
87-99
Average (%)
94
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.54
522.11
1502.52
229.29
514.93
1370.89
Standard deviation
14.51
21.09
70.17
14.03
29.87
46.06
C V (%)
6.58
4.04
4.67
6.12
5.8
3.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ANGPTL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ANGPTL3 in samples. No significant cross-reactivity or interference between Rat ANGPTL3 and analogues was observed.
