The Rat apoA1 (Apolipoprotein A1) CLIA Kit is specifically designed for the quantitative detection of Apolipoprotein A1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Apolipoprotein A1 is an important component of high-density lipoprotein (HDL) and plays a key role in lipid metabolism and cardiovascular health.
Abnormal levels of Apolipoprotein A1 have been associated with various cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat apoA1 (Apolipoprotein A1) CLIA Kit is a reliable tool for researchers looking to investigate the role of Apolipoprotein A1 in physiological and pathological processes related to cardiovascular health in rat models.
Product Name:
Rat ApoA1 (Apolipoprotein A1) CLIA Kit
SKU:
RTES00610
Target:
Rat ApoA1 (Apolipoprotein A1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ApoA1. You can calculate the concentration of Rat ApoA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
91-106
95-112
Average (%)
105
97
103
1:4
Range (%)
96-111
99-115
85-99
Average (%)
102
107
91
1:8
Range (%)
96-110
98-113
96-108
Average (%)
102
106
102
1:16
Range (%)
85-97
92-103
90-101
Average (%)
92
97
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.68
53.68
181.77
22.0
57.04
196.28
Standard deviation
1.78
5.0
12.36
2.57
5.4
16.98
C V (%)
8.21
9.31
6.8
11.68
9.47
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ApoA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoA1 in samples. No significant cross-reactivity or interference between Rat ApoA1 and analogues was observed.
