The Rat APOE (Apolipoprotein E) CLIA Kit is specially designed for the quantitative measurement of APOE levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of research applications.APOE is a critical protein involved in lipid metabolism and transportation, playing a crucial role in cardiovascular health and neurodegenerative diseases. By measuring APOE levels in rat samples, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
Overall, the Rat APOE CLIA Kit from Assay Genie is a valuable tool for researchers studying cardiovascular health, neurodegenerative disorders, and lipid metabolism in rat models. With its high performance and ease of use, this kit is an essential addition to any laboratory conducting research in these areas.
Product Name:
Rat ApoE (Apolipoprotein E) CLIA Kit
SKU:
RTES00616
Target:
Rat ApoE (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ApoE. You can calculate the concentration of Rat ApoE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
104-116
87-102
Average (%)
98
110
93
1:4
Range (%)
95-110
100-113
94-108
Average (%)
100
107
102
1:8
Range (%)
90-101
97-111
88-103
Average (%)
95
103
95
1:16
Range (%)
86-100
90-101
93-106
Average (%)
91
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.97
4.67
13.9
1.95
4.51
13.23
Standard deviation
0.18
0.42
1.19
0.17
0.46
1.16
C V (%)
9.14
8.99
8.56
8.72
10.2
8.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ApoE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoE in samples. No significant cross-reactivity or interference between Rat ApoE and analogues was observed.
