Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit
The Rat B-Cell CLL/Lymphoma 2-Like Protein 2 (BCL2L2) ELISA Kit is a specialized assay designed for the accurate quantification of BCL2L2 levels in rat biological samples such as serum, plasma, and tissue homogenates. This ELISA kit offers high sensitivity and specificity, allowing researchers to obtain reliable and reproducible results for their experiments.BCL2L2 is a key protein involved in regulating apoptosis and cell survival, making it a pivotal player in various cellular processes and diseases. Dysregulation of BCL2L2 expression has been linked to cancer, autoimmune disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target and diagnostic marker.
The Rat BCL2L2 ELISA Kit is a valuable tool for researchers studying the role of BCL2L2 in disease pathogenesis and for those developing novel therapeutic strategies targeting this protein. With its user-friendly protocol and high-performance characteristics, this ELISA kit is an essential addition to any laboratory conducting research in the field of molecular biology and cell biology.
Product Name:
Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit
Product Code:
RTDL00136
Size:
96 Assays
Target:
Bcl2L2
Synonyms:
B-Cell CLL/Lymphoma 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
