Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit
The Rat BDNF (Brain-Derived Neurotrophic Factor) CLIA Kit is specifically designed for the precise measurement of BDNF levels in rat samples. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for various research purposes.BDNF is a key neurotrophic factor that plays a vital role in neuronal survival, growth, and differentiation. It is closely linked to various neurological and psychiatric disorders, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
With its reliable performance and ease of use, the Rat BDNF CLIA Kit is an indispensable tool for researchers looking to investigate BDNF signaling pathways, neurodevelopmental processes, and potential treatment strategies.
Product Name:
Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit
SKU:
RTES00618
Target:
Rat BDNF (Brain Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
86-98
97-112
Average (%)
105
92
103
1:4
Range (%)
86-102
97-113
93-105
Average (%)
93
105
98
1:8
Range (%)
92-105
94-108
89-104
Average (%)
97
101
96
1:16
Range (%)
104-118
92-106
92-107
Average (%)
110
97
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
101-114
107
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.41
75.46
354.29
42.97
73.19
351.39
Standard deviation
4.27
7.93
31.43
5.46
6.8
38.23
C V (%)
10.83
10.51
8.87
12.71
9.29
10.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
