Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
The Rat bFGF (FGF2) Basic Fibroblast Growth Factor CLIA Kit is a highly sensitive and reliable assay designed for the accurate measurement of basic fibroblast growth factor (bFGF or FGF2) levels in rat samples including serum, plasma, and cell culture supernatants. bFGF (FGF2) is a potent growth factor that plays a crucial role in cell proliferation, migration, and differentiation, making it an essential regulator of various biological processes. This kit provides researchers with the necessary tools to study the impact of bFGF (FGF2) on cell growth and development, as well as its potential implications in diseases such as cancer, cardiovascular disorders, and neurological conditions.
With its high specificity and reproducibility, the Rat bFGF (FGF2) Basic Fibroblast Growth Factor CLIA Kit is an invaluable tool for investigating the role of bFGF (FGF2) in physiological and pathological processes, ultimately leading to a better understanding of disease mechanisms and the development of potential therapeutic interventions.
Product Name:
Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
SKU:
RTES00056
Target:
Rat bFGF/FGF2 (Basic Fibroblast Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat bFGF/FGF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat bFGF/FGF2. You can calculate the concentration of Rat bFGF/FGF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
95-110
93-107
Average (%)
108
101
100
1:4
Range (%)
89-101
100-114
97-110
Average (%)
94
108
104
1:8
Range (%)
88-101
101-119
85-96
Average (%)
94
109
91
1:16
Range (%)
93-110
98-112
95-112
Average (%)
100
105
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
102
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.02
47.86
219.1
24.46
52.45
238.76
Standard deviation
2.4
4.16
20.07
2.0
5.29
20.34
C V (%)
9.22
8.69
9.16
8.18
10.09
8.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat bFGF/FGF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat bFGF/FGF2 in samples. No significant cross-reactivity or interference between Rat bFGF/FGF2 and analogues was observed.
