The Rat BMG Beta2-mg (Beta-2 Microglobulin) CLIA Kit is a high-quality assay designed for the accurate measurement of beta-2 microglobulin levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.Beta-2 microglobulin is a vital protein involved in various physiological processes, including immune response and kidney function. Elevated levels of beta-2 microglobulin have been linked to conditions such as kidney disease, autoimmune disorders, and certain types of cancer, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.
With its user-friendly protocol and robust performance, the Rat BMG Beta2-mg CLIA Kit is an indispensable tool for researchers seeking to investigate the role of beta-2 microglobulin in health and disease in rat models. Visit the provided URL for more information and to order your kit today.
Product Name:
Rat BMG/ beta2-MG (Beta-2-Microglobulin) CLIA Kit
SKU:
RTES00600
Target:
Rat BMG/ beta2-MG (Beta-2-Microglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/β2-MG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMG/β2-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMG/β2-MG. You can calculate the concentration of Rat BMG/β2-MG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-108
97-112
92-102
Average (%)
105
104
96
1:4
Range (%)
98-111
99-111
97-106
Average (%)
104
104
100
1:8
Range (%)
87-102
93-107
90-99
Average (%)
95
100
94
1:16
Range (%)
97-108
94-100
89-97
Average (%)
103
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
94-103
98
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.51
435.89
1703.02
183.7
453.66
1879.3
Standard deviation
10.36
19.83
74.08
10.34
20.82
86.82
C V (%)
5.38
4.55
4.35
5.63
4.59
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMG/β2-MG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMG/β2-MG in samples. No significant cross-reactivity or interference between Rat BMG/β2-MG and analogues was observed.
